We demonstrate that rigid anchoring of fluorescent proteins through double tagging (FPs) in living cells can significantly enhance the contrast in fluorescence polarization microscopy (FPM) by locking ...

Fluorescence microscopy is indispensable for visualizing biological structures and dynamics, yet its efficiency is limited—over half of emitted photons fall outside the objective’s numerical aperture ...

Multiphoton microscopy is used in biomedical research to study cells and tissues. Today, so-called two-photon microscopy is used to study processes within cells, but the technique has limitations in ...

A new two-photon fluorescence microscope developed at UC Davis can capture high-speed images of neural activity at cellular resolution thanks to a new adaptive sampling scheme and line illumination.

In recent years, fluorescence quenching microscopy (FQM) 1-3 has emerged as a viable technique that allows for the swift, cost-effective, and accurate imaging of two-dimensional (2D) materials like ...

Bacteriophages, or phages, viruses that selectively target and infect bacteria, have drawn growing attention for their potential use in a host of biotechnological processes to benefit humankind, from ...

ST. LOUIS, MO, August 20, 2025 - A team of expert scientists led by Kirk Czymmek, PhD, director of the Advanced Bioimaging Laboratory at the Donald Danforth Plant Science Center and Heather E.

Tunable Bessel beam two-photon fluorescence microscopy enables high-speed volumetric intravital imaging of subcellular dynamics within living mouse brains with fully tunable spatial resolution and ...

Comparing Microscopy Techniques: This image contrasts conventional fluorescent microscopy with STORM processing, highlighting how STORM achieves superior resolution by activating and precisely ...

Microscopy is an imaging technique that enables us to see a world that would otherwise be invisible to us. Once upon a time, visualizing cells, microbes and other entities not perceptible to the naked ...

Cryo-electron microscopy (cryo-EM) can help scientists determine the three-dimensional structure of proteins in unprecedented ...

Deblurring by pixel reassignment remaps raw fluorescent microscopy images to sharpen images via pixel reassignment. Credit: Zhao and Mertz, doi 10.1117/1.AP.5.6.066004. Obtaining high-resolution ...