Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration
This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” ...
This is a preview. Log in through your library . Abstract Exposure of corn seedlings to different concentrations of the toxic heavy metal mercury(II) led to significantly altered activities of the ...
ATM stands for ataxia telangiectasia mutated, a gene that contains information for synthesizing a protein that controls the rate of cell growth. When the strands of DNA in a cell become damaged, the ...
The loss of catalytic activity (irreversible denaturation) of α -chymotrypsin (EC 3.4.21.1) as a function of pH between 6 and 13 is described. Around neutrality the irreversible denaturation is second ...
pH is a measure of how acidic or alkaline a solution is. The diagram below shows what happens to an enzyme when denaturation occurs. Each enzyme has an optimum pH but it also has a working range of pH ...
This causes the formation of more enzyme-substrate complexes, leading to an increase in enzyme activity. This means the active site loses its important shape and can no longer form enzyme-substrate ...
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