Lysates were clarified by centrifugation, filtered through a 0.45 μm filter and supplemented ... 20 mM HEPES 7.5) on an AKTA system. Pooled eluted fractions were dialyzed twice in 5 L of Milli-Q water ...

For the determination of the iron content, the suspension was diluted 1:25 in deionized H 2 O (produced in-house via the Merck Milli-Q ® Direct water purification system, Darmstadt, Germany) and ...

For all the analyses, ultrapure water was purified using an Elix 3 coupled to a Milli-Q Gradient A10 system (Millipore, Bedford, MA, USA). All solutions were filtered through a 0.22 μm membrane filter ...

Mackie and colleagues present a valuable comparison of lateralized gustation in two well-studied nematodes. The evidence they present that ASEL/R lateralization exists and is achieved by different ...

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1 Ethiopian Institute of Water Resources, Addis Ababa University ... applying natural wastewater purification methods such as wetlands, and soil phytoremediation for grossly contaminated farms.