Frontiers20d
Temperature and Nutrient Limitations Decrease Transfer of Conjugative IncP-1 Plasmid pKJK5 to Wild Escherichia coli Strains
Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically ...
Frontiers21d
The G285S mutation in nsP1 is sufficient to render Sindbis virus as a stable vector for gene delivery
Based on this mutation, we constructed a new vector for the applications in neural circuits mapping ... When BHK-21 cells are 80–90% confluent, they are ready for transfection. The plasmid was ...
The EMBO Journal28d
The NLRP1 inflammasome is an essential and selective mediator of axon pruning in neurons
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Axon pruning is a unique process neurons ...
The EMBO Journal29d
Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The prevailing view on post-translational ...
eLife4mon
APP β-CTF triggers cell-autonomous synaptic toxicity independent of Aβ
For plasmid transfection ... We inserted β-CTF-flag and GFP into the pFHTrePW vector (Ni et al., 2023) backbone under the control of the Tet Response Element (TRE 3G). The rtTA for the tet-off system ...
eLife1y
Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells
The SV40 polyA signal was amplified from pMAX-GFP and ligated to the digested plasmid to generate ... vector to generate an intermediate vector. The intermediate vector was digested with AgeI and an ...